Use of colorimetric culture methods for detection of Mycobacterium tuberculosis complex isolates from sputum samples in resource-limited settings - Boum, Yap; Orikiriza, Patrick; Rojas-Ponce, G.; Riera-Montes, M.; Atwine, D.; Nansumba, Margaret; Bazira, J.; Tuyakira, E.; Debeaudrap, Pierre; Bonnet, Maryline; Page, A. L. (J Clin Microbiol, 2013)
Despite recent advances, tuberculosis (TB) diagnosis remains imperfect in resource-limited settings due to its complexity and costs, poor sensitivity of available tests, or long times to reporting. We present a report on the use of colorimetric methods, based on the detection of mycobacterial growth using colorimetric indicators, for the detection of Mycobacterium tuberculosis in sputum specimens. We evaluated the nitrate reductase assay (NRA), a modified NRA using para-nitrobenzoic acid (PNB) (NRAp), and the resazurin tube assay using PNB (RETAp) to differentiate tuberculous and nontuberculous mycobacteria. The performances were assessed at days 18 and 28 using mycobacterium growth indicator tube (MGIT) and Lowenstein-Jensen (LJ) medium culture methods as the reference standards. We enrolled 690 adults with suspected pulmonary tuberculosis from a regional referral hospital in Uganda between March 2010 and June 2011. At day 18, the sensitivities and specificities were 84.6% and 90.0% for the NRA, 84.1% and 92.6% for the NRAp, and 71.2% and 99.3% for the RETAp, respectively. At day 28, the sensitivity of the RETAp increased to 82.6%. Among smear-negative patients with suspected TB, sensitivities at day 28 were 64.7% for the NRA, 61.3% for the NRAp, and 50% for the RETAp. Contamination rates were found to be 5.4% for the NRA and 6.7% for the RETAp, compared with 22.1% for LJ medium culture and 20.4% for MGIT culture. The median times to positivity were 10, 7, and 25 days for colorimetric methods, MGIT culture, and LJ medium culture,respectively. Whereas the low specificity of the NRA/NRAp precludes it from being used for TB diagnosis, the RETAp might provide an alternative to LJ medium culture to decrease the time to culture results in resource-poor settings.
00010 Journal of clinical microbiology
Keywords: journalArticle; Adult; Colorimetry/*methods; Female; Humans; Male; Middle Aged; Mycobacterium tuberculosis/growth & development/*isolation &; Nitrate Reductase/metabolism; Nitrobenzoates/metabolism; Oxazines/metabolism; Sensitivity and Specificity; Sputum/*microbiology; Time Factors; Tuberculosis/*diagnosis/microbiology; Uganda; Xanthenes/metabolism; developing countries; purification/metabolism